| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206132 |
| Chromosome: | chromosome 7 |
| Location: | 2949519 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332851 | SELM2,SELENOM,SELM | (1 of 1) PTHR13077:SF7 - SELENOPROTEIN M; Selenoprotein M | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGCACATATACTCCCTACCTGGGTCA |
| Internal bar code: | GCCGCCTGAGTTCGCATAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 98.3114 |
| LEAP-Seq n confirming: | 2387 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGCCCAACATCAT |
| Suggested primer 2: | AACACCTGGGTGAAGTCGTC |