| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206166 |
| Chromosome: | chromosome 4 |
| Location: | 1568491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213400 | SUB7 | (1 of 1) PTHR10795//PTHR10795:SF419 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // SUBFAMILY NOT NAMED; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGACCAGCAGTGAGTATGTGAATCAT |
| Internal bar code: | TGAGTTGAGGGCCTGACGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.7448 |
| LEAP-Seq n confirming: | 3908 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTGTGCTTGTGTCTGTG |
| Suggested primer 2: | AACAGGAACCAACATGAGCC |