Insertion junction: LMJ.RY0402.206174_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.206174
Chromosome:chromosome 12
Location:8323565
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g549700 (1 of 1) K11884 - RNA-binding protein PNO1 (PNO1, DIM2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAATACAATGAACCGCGATCTGTGCGTGA
Internal bar code:GCGGGCCGTGCTAATTACGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:342
LEAP-Seq percent confirming:99.3648
LEAP-Seq n confirming:3598
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGCTGACTGACAACGTGC
Suggested primer 2:CTTGACTACCTGGCGTGACA