| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206192 |
| Chromosome: | chromosome 12 |
| Location: | 2849838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502250 | PLP4,PLAP4 | Plastid lipid associated protein 4; (1 of 17) PF04755 - PAP_fibrillin (PAP_fibrillin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGCAACACACACGACACGGCGTACTG |
| Internal bar code: | GCCACACCATGAGACTCTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 98.3425 |
| LEAP-Seq n confirming: | 712 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAATTACGCACGCACTC |
| Suggested primer 2: | TAGGGAATGCATGGAGGAAG |