| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206202 |
| Chromosome: | chromosome 13 |
| Location: | 1853734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575366 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGGTCGGGGCCCACCGCATCCGCTTTG |
| Internal bar code: | GCGTGTACGCCCCGAGAGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 97.8648 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTACTCGCTCCACTTCCG |
| Suggested primer 2: | CTAGCAGCAGTAGCACGACG |