| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206208 |
| Chromosome: | chromosome 12 |
| Location: | 7718089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553950 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGAAGGGGTGGCGCGGCGGAGGGGTGG |
| Internal bar code: | AGGCTGAGTTACGGCTGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 98.7983 |
| LEAP-Seq n confirming: | 7235 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCCAGAGGCAATAAGC |
| Suggested primer 2: | ACTCACTGGCTACACACCCC |