| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206213 |
| Chromosome: | chromosome_3 |
| Location: | 4790128 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178500 | RIB43,RIB43A | Flagellar protofilament ribbon protein | antisense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACAGCAGGCAGGCGCGCCGAGGACGCCC |
| Internal bar code: | GGGAGCATCGAAGGGACTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.3168 |
| LEAP-Seq n confirming: | 2326 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCTTCTACCTTCTGCG |
| Suggested primer 2: | GTGGTGCGGAGAAATGAGAT |