| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206221 |
| Chromosome: | chromosome 14 |
| Location: | 3364100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630150 | (1 of 1) PTHR10131//PTHR10131:SF52 - TNF RECEPTOR ASSOCIATED FACTOR // TNF RECEPTOR-ASSOCIATED FACTOR FAMILY PROTEIN DDB_G0268444-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGAACCAGTGGTGTGGGGCGGGTTTAT |
| Internal bar code: | CTGCGGTGGGATCCTTGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCGTACACAACGTCAAC |
| Suggested primer 2: | AGGAGATGGCCAAGGAATTT |