| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206252 |
| Chromosome: | chromosome 16 |
| Location: | 113633 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695400 | (1 of 2) PF09733 - VEFS-Box of polycomb protein (VEFS-Box) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGACAGGCGCAGAAGCATTAAGTGCCT |
| Internal bar code: | ATCAGTTCCTTCCCTGACAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.7423 |
| LEAP-Seq n confirming: | 3870 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATGGGGTGATCAAGGG |
| Suggested primer 2: | CCATCCAATCCCAACATACC |