| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206272 |
| Chromosome: | chromosome 6 |
| Location: | 5944131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288700 | GYD1 | Glycolate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTCCCCTCCCCACACCGCCCCACAGG |
| Internal bar code: | CGCAGTCCGGCAGCTACGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 98.3051 |
| LEAP-Seq n confirming: | 2146 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAAGACCAGCCACCATC |
| Suggested primer 2: | CACAAGGGGTGTTCAGTGTG |