| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206281 |
| Chromosome: | chromosome 10 |
| Location: | 1415385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428150 | (1 of 1) PTHR10566:SF58 - PROTEIN D2023.6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCCTCTTGGTGTTGCCTCCGCCCCGCCC |
| Internal bar code: | GGGACTCCACGGCTGCGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 96.8774 |
| LEAP-Seq n confirming: | 1241 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCCTTGCAACTTCTTCT |
| Suggested primer 2: | GGCGAGAAGTAGGCAAACAG |