| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206293 |
| Chromosome: | chromosome 12 |
| Location: | 3516087 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513100 | (1 of 1) 3.2.1.165 - Exo-1,4-beta-D-glucosaminidase / GlcNase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCTCCGTCAGCCCCGAGCCGCCGCT |
| Internal bar code: | CGCTGGCCTACCGGGAAGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 86.5217 |
| LEAP-Seq n confirming: | 199 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATGAATGAACGTCGTG |
| Suggested primer 2: | GTTTCCAGCACTAAGCAGGC |