| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206294 |
| Chromosome: | chromosome 14 |
| Location: | 1109032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615100 | MSR1,MSRB1B | (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB); Methionine sulphoxide reductase 1B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCACGTGCTCTCCGGCGTGTCTTTCCCT |
| Internal bar code: | AATCTCGGCACAGTCGAGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.7468 |
| LEAP-Seq n confirming: | 6696 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCACAGGAAGTGCGTAT |
| Suggested primer 2: | ACGCGATGTCGAGTCTTCTT |