Insertion junction: LMJ.RY0402.206302_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.206302
Chromosome:chromosome 16
Location:6323085
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g674450 (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAAGGGAAAGTGAAGGCACCCCTGCGCC
Internal bar code:CCGTATCGGGGCTGATCTAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:889
LEAP-Seq percent confirming:97.7273
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCTCTTCAATATTGCGCT
Suggested primer 2:TCGCTACTGCCACTCATCAC