| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206328 |
| Chromosome: | chromosome 2 |
| Location: | 2459500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091900 | FAP301 | Flagellar Associated Protein 301 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCCCAAGCACACACCCACAGCCTCAC |
| Internal bar code: | ATCCCTGCTCCTGCAGGACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.262 |
| LEAP-Seq n confirming: | 9415 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCTCCTCAACGTCACC |
| Suggested primer 2: | ATTTTGCTGGGTGGAAAGTG |