| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206378 |
| Chromosome: | chromosome_6 |
| Location: | 8413082 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g307012 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCGTTTGGGAAAGGGGTCCGTGCCTTCGG |
| Internal bar code: | ACTGAATGGCGCAAGGCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.5526 |
| LEAP-Seq n confirming: | 6675 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGCCAGGTCTAGCTCC |
| Suggested primer 2: | GCAAAAGTGGCTCACACTCA |