| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206387 |
| Chromosome: | chromosome 11 |
| Location: | 1480541 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467741 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATGCGATGGACGAGTGGGCGGAGGAGGA |
| Internal bar code: | GTGTACGCTCGAGCGGGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 99.6096 |
| LEAP-Seq n confirming: | 1531 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAAAGCAACAACCGGAC |
| Suggested primer 2: | GCTTTCCTGCTGGTGTAAGC |