Insertion junction: LMJ.RY0402.206395_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.206395
Chromosome:chromosome 12
Location:6003436
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g535019 (1 of 139) IPR029787 - Nucleotide cyclase 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGCAAAGGTCACAGACGTCCAGCAGCGC
Internal bar code:CTACGACTGGGGGGGAACATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:99.7089
LEAP-Seq n confirming:1370
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACAAGGACCCTCTCAAG
Suggested primer 2:CAAACATGTCTCTGCGCACT