| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206414 |
| Chromosome: | chromosome 17 |
| Location: | 4110844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729500 | (1 of 1) PTHR22870//PTHR22870:SF136 - REGULATOR OF CHROMOSOME CONDENSATION // ALSIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCAACTTGAGCGTCCAGAACACGACC |
| Internal bar code: | CAGGACCTAGACATTCCGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.7955 |
| LEAP-Seq n confirming: | 488 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCAAATCCCCCTTACT |
| Suggested primer 2: | TCATGCATCAAATTGCGTTT |