| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206426 |
| Chromosome: | chromosome 12 |
| Location: | 7531624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555750 | CPK7 | (1 of 13) PTHR10584 - SUGAR KINASE; putative carbohydrate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTACCCGTAGCCAATTTTGTAGTGGCT |
| Internal bar code: | GGGTCGGCATGCACTTGGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 718 |
| LEAP-Seq percent confirming: | 99.6627 |
| LEAP-Seq n confirming: | 5909 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGGTTCCCGTTAGCACT |
| Suggested primer 2: | GAGATGTGGCGTTCTGGATT |