Insertion junction: LMJ.RY0402.206431_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.206431
Chromosome:chromosome 17
Location:2336911
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g714400 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGTCCACAACCCACACGCAGCTTCACC
Internal bar code:CATGCTACGTTGTGACTCCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1283
LEAP-Seq percent confirming:98.9198
LEAP-Seq n confirming:1282
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGAACATCCTGCTGAACG
Suggested primer 2:ACTTCCCCTGTTCACAATGC