| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206442 |
| Chromosome: | chromosome_6 |
| Location: | 7196740 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g297850 | DHC12 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCAGCCTGCCGCCCGAGGGCAGTGTGTAC |
| Internal bar code: | CTGCCAAACGCCGTTGGGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 11 |
| LEAP-Seq percent confirming: | 9.09091 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCACAGCGTCTTTGTC |
| Suggested primer 2: | GTCATCGTCGTCGTCATCTG |