| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206442 |
| Chromosome: | chromosome 13 |
| Location: | 2130920 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577900 | (1 of 1) PF13865 - C-terminal duplication domain of Friend of PRMT1 (FoP_duplication) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCATGATTCCCGGTAAGCGCTCTGCCCG |
| Internal bar code: | CGTAGCACCTCGGCAAGGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 94.7826 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGCAGTCAGTGCAGCG |
| Suggested primer 2: | TCACGTGCAGGCTGATCTAC |