| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206455 |
| Chromosome: | chromosome 2 |
| Location: | 5584480 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109000 | (1 of 2) PF03254 - Xyloglucan fucosyltransferase (XG_FTase) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGCTGGCGGCCGCACGTCCAGTCTAT |
| Internal bar code: | GGCGTGCCCGCGTAGGGCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3 |
| LEAP-Seq percent confirming: | 88.8444 |
| LEAP-Seq n confirming: | 1776 |
| LEAP-Seq n nonconfirming: | 223 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCAGTAGGCCACGTCAC |
| Suggested primer 2: | AGTAGCCTTGCTGCTTCTGG |