| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206458 |
| Chromosome: | chromosome_11 |
| Location: | 1542340 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467753 | HMO2 | Heme oxygenase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGACGTGCTCAAGGGCGGGCTGTACCGG |
| Internal bar code: | CAAGGCAGCAATTAGTAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.2321 |
| LEAP-Seq n confirming: | 9692 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTCTGTTCTCCGACA |
| Suggested primer 2: | GAGAACACGTACGCAAGCAA |