| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206458 |
| Chromosome: | chromosome 11 |
| Location: | 1542410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467753 | HMOX2,HMO2 | Heme oxygenase; (1 of 1) PTHR10720 - HEME OXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTATGCGCGCGGAGCTTAAACGTGCACG |
| Internal bar code: | ACACACAACAGTGGCCGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.682 |
| LEAP-Seq n confirming: | 3762 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCGTACATCGTGTGTC |
| Suggested primer 2: | GAGAACACGTACGCAAGCAA |