| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206470 |
| Chromosome: | chromosome 10 |
| Location: | 3357218 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443900 | BOR1 | (1 of 1) PF00955 - HCO3- transporter family (HCO3_cotransp); Borate transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAACACCCCAACAGCTCTCGTGCACAG |
| Internal bar code: | ATGGCTTAAGAAGGACATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.7049 |
| LEAP-Seq n confirming: | 4054 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCCGGTGCTATCTAC |
| Suggested primer 2: | CATCACCACACATCACCACA |