| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206480 |
| Chromosome: | chromosome 8 |
| Location: | 402331 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358576 | (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCAACGGCCCACCCGCCTGCCGCGCAA |
| Internal bar code: | AGGGGCGGTGCCGTAGACATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 98.5816 |
| LEAP-Seq n confirming: | 6672 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTTCAGACATTAGCCC |
| Suggested primer 2: | GACGGAGGACTACCTGGTGA |