| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206491 |
| Chromosome: | chromosome 8 |
| Location: | 1246943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363300 | (1 of 1) 2.4.2.1//2.4.2.28 - Purine-nucleoside phosphorylase / PNPase // S-methyl-5'-thioadenosine phosphorylase / MTAPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAATGCAAACTTCCCAACGGACGTGGAG |
| Internal bar code: | GGTATGGGCTGGGTATATAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.6685 |
| LEAP-Seq n confirming: | 11123 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGAACCATGCTTTGATA |
| Suggested primer 2: | CGGGAGTGTCCAGAAATGTT |