| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206520 |
| Chromosome: | chromosome 10 |
| Location: | 1287453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427300 | PF24,RSP2 | Radial Spoke Protein 2; (1 of 1) PTHR23356//PTHR23356:SF5 - DPY30-RELATED // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTTTATACAAAAACGATCATTGCACGT |
| Internal bar code: | TGAGTCAGGGCGATAGCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 99.1211 |
| LEAP-Seq n confirming: | 2030 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTTGACTTGGTGGGTGC |
| Suggested primer 2: | CGCTAGAGGACAGGAAGTGG |