| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206557 |
| Chromosome: | chromosome 13 |
| Location: | 2551986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580700 | DAL2 | allantoate deiminase; (1 of 1) 3.5.3.9 - Allantoate deiminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTTACATTACAAGATGGACGAACAGGC |
| Internal bar code: | TCGTGCCGTGGCGCGGAGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.8015 |
| LEAP-Seq n confirming: | 6535 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCGAACGCACACACAAG |
| Suggested primer 2: | GTTGAGATCGGGAACGAAAA |