| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206558 |
| Chromosome: | chromosome 9 |
| Location: | 4251430 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394510 | (1 of 1) IPR002044//IPR010989//IPR013784 - Carbohydrate binding module family 20 // t-SNARE // Carbohydrate-binding-like fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCATGATAGCGTGTGTACCTCATCTGT |
| Internal bar code: | CACTCCCCTTTCGTGCGCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 41 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAGTTTGGTCCCCGAAT |
| Suggested primer 2: | GCGGCAGTACCGTATTTGAT |