| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206559 |
| Chromosome: | chromosome 9 |
| Location: | 3387599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388838 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTTGTTATGCCTTTGAAGGGTAGGGA |
| Internal bar code: | TGGGTTTATGTATCGCAAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 98.2394 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAATGGAGATGTGCCACGA |
| Suggested primer 2: | TGACTGGTTGCAGAATGCTC |