| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206561 |
| Chromosome: | chromosome 2 |
| Location: | 5693797 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110000 | (1 of 1) PF07974//PF13920 - EGF-like domain (EGF_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATCCGTTGCCTCACGAACAGCCGAGG |
| Internal bar code: | ACAAGTGTGCGTGTTCGTTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCACGACAGGATAGGTGA |
| Suggested primer 2: | GCATTATGGGGGACAACATC |