| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206584 |
| Chromosome: | chromosome 5 |
| Location: | 774833 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCATGCTTCCGCTCTTCTTGCAAGCAGC |
| Internal bar code: | TCGCGTACCCTTCTACGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.8242 |
| LEAP-Seq n confirming: | 5679 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGGGCGTTTTTGCTTAG |
| Suggested primer 2: | GAGCTGTCAACAGACGGTGA |