| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206584 |
| Chromosome: | chromosome 10 |
| Location: | 1678168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430050 | UBC20 | E2 Ubiquitin conjugating enzyme; (1 of 1) K10583 - ubiquitin-conjugating enzyme E2 S (UBE2S, E2EPF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCATGACCCGACACGTAGTTGCAAGC |
| Internal bar code: | TACTGGACGGTGATTTTATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.7443 |
| LEAP-Seq n confirming: | 2731 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGGTGAAGATTGAGAAG |
| Suggested primer 2: | GCTGCTGGAGGACTATGAGG |