| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206585 |
| Chromosome: | chromosome 3 |
| Location: | 6648321 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197050 | HTV2 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAATACATGAAGGTGCGGCAGCTCAC |
| Internal bar code: | TGTCCCGACTAAATAGAAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.4382 |
| LEAP-Seq n confirming: | 4779 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAACCTGCGACATAGCTG |
| Suggested primer 2: | ATGAGCAGCTCCGTTGTCTT |