Insertion junction: LMJ.RY0402.206610_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.206610
Chromosome:chromosome 7
Location:3023699
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g333400 MMP23 Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCAACAGGACTTCTGCGACAACCTCATC
Internal bar code:GACCGTTGGTAATCCCTCGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:99.5667
LEAP-Seq n confirming:1149
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCCAGCAAGTACAGTGA
Suggested primer 2:CCGGTGGAAATTCTTCTTCA