| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206614 |
| Chromosome: | chromosome 16 |
| Location: | 6520779 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672750 | (1 of 2) K14944 - RNA-binding protein Nova (NOVA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCAACCGTTGACCACGCATGGAGGGAC |
| Internal bar code: | GAACTATGGGACATGCCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 99.1667 |
| LEAP-Seq n confirming: | 714 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACACGCACCCTAACACTT |
| Suggested primer 2: | CTAGGCGCAAACTCAACACA |