| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206646 |
| Chromosome: | chromosome 3 |
| Location: | 726775 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146087 | (1 of 3) 1.14.11.33 - DNA oxidative demethylase / Alkylated DNA repair protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACATACCGACATACGGGATGCACGATTC |
| Internal bar code: | TTCGTTCGCGGATTCTGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 85.4839 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTACCCATGCTGCCTCTC |
| Suggested primer 2: | AGGATGGGTCCAAGTGAGTG |