| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206652 |
| Chromosome: | chromosome 9 |
| Location: | 3417509 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389060 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCGCTCAGTGTTGCTTGGGGCACCGG |
| Internal bar code: | GCCGGCGAGTAGGACACGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 93.6919 |
| LEAP-Seq n confirming: | 9981 |
| LEAP-Seq n nonconfirming: | 672 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCCTGTATTTCACCTTG |
| Suggested primer 2: | GTGCTGTTGGCGAGTAGTGA |