Insertion junction: LMJ.RY0402.206665_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.206665
Chromosome:chromosome 10
Location:6361538
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g465550 CLPD1,CLPD (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; ClpD chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCGCACTACCTCCTACGCCCTGCCTCC
Internal bar code:CAGTCGTCGGGGAGGAGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:120
LEAP-Seq percent confirming:99.2553
LEAP-Seq n confirming:933
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACGAAGTGAAATCGGGG
Suggested primer 2:CATCGTGTCTCGCTCTGTGT