| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206680 |
| Chromosome: | chromosome 3 |
| Location: | 4491770 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176200 | (1 of 1) PTHR22904//PTHR22904:SF364 - TPR REPEAT CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAGCGCAAGCTGCGCGCTCGGGCGATG |
| Internal bar code: | CAGTACAACCAGAAGCCCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.7957 |
| LEAP-Seq n confirming: | 4885 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTTGGCCTTGAATGTCT |
| Suggested primer 2: | AAGAAGTCCGCAATGGACAG |