| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206699 |
| Chromosome: | chromosome 16 |
| Location: | 4764150 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGACACGCCGCGCAGACCGACCCCTC |
| Internal bar code: | GGCGGGTAGTCTCCGCGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 7.55754 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 2691 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACTCACGACAAGCCT |
| Suggested primer 2: | GATCTATGTGGTGGATCCCG |