| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206703 |
| Chromosome: | chromosome 1 |
| Location: | 6650482 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047650 | (1 of 1) K10268 - F-box and leucine-rich repeat protein 2/20 (FBXL2_20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCACACACAGGCTGCGATGGCTTTCC |
| Internal bar code: | GTCCGCGGTGCGACACGCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.492 |
| LEAP-Seq n confirming: | 2350 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGCAGGTATGGCTAGG |
| Suggested primer 2: | TACAGGCAAGAGCAGAGGGT |