| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206718 |
| Chromosome: | chromosome 11 |
| Location: | 1296282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467711 | (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAAGGGAGGGAGGAGTGTTGGGGCACG |
| Internal bar code: | CGCTGTCCGGCTCAACCACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.0505 |
| LEAP-Seq n confirming: | 20968 |
| LEAP-Seq n nonconfirming: | 201 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGAGGTCAGTTGATGGC |
| Suggested primer 2: | TGCTCGTAGGCATAATGCTG |