| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206780 |
| Chromosome: | chromosome 2 |
| Location: | 4703163 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101800 | ARFB1,ARFB | Peptide chain release factor/ribosome rescue factor; (1 of 1) K15033 - peptidyl-tRNA hydrolase ICT1 [EC:3.1.1.29] (ICT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCGCCGTCCAGCCGGTCGCTTTTTGAT |
| Internal bar code: | CCCGTCGGGGGGATCGGGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 99.785 |
| LEAP-Seq n confirming: | 5570 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGATGTGGTAGAAGGGT |
| Suggested primer 2: | ACACCTCTCCCTCCCTCACT |