| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206793 |
| Chromosome: | chromosome 6 |
| Location: | 1084446 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256650 | (1 of 4) PTHR10887//PTHR10887:SF331 - DNA2/NAM7 HELICASE FAMILY // HELICASE MOV10L1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGTGAGCTGTTGTTGTTGGTGCATCA |
| Internal bar code: | GCTTTTTGTTTTGTTATACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.7038 |
| LEAP-Seq n confirming: | 1010 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTTGCAGCATCAGGAA |
| Suggested primer 2: | TACAGCTGTGGGTGTTCAGC |