| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206803 |
| Chromosome: | chromosome 5 |
| Location: | 319132 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGCCCAGCTGTCTATCTAACCCACAGC |
| Internal bar code: | AAGGGGACACATGTGCACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 99.7314 |
| LEAP-Seq n confirming: | 22651 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GCTTGCTGTATGTTTGGGGT |