Insertion junction: LMJ.RY0402.206814_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.206814
Chromosome:chromosome 17
Location:4191433
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g730100 RSE1,RSEP1 (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGATAACGCACGCACGCCCGTGCTTGC
Internal bar code:CCACAGGATGTATTGCACGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:459
LEAP-Seq percent confirming:57.3684
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:81
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGATACGAGACGCTGGTGGT
Suggested primer 2:CAAGGGCGCGTGTATAAAAT